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Advanced course

Title: Chlorophyll Quantification: Standardized Methods according to Standard Methods

Teaching plan

- Introduction to chlorophyll and its importance

- Types of chlorophylls (Chlorophyll-a, Chlorophyll-b, Chlorophyll-c, pheophytin)

- Applications of chlorophyll quantification in environmental studies and water quality monitoring

- Comparison between different extraction and quantification methods

- Choosing the appropriate solvent

- Laboratory procedures for efficient extraction

- Impact of storage conditions and extraction time

- Quantification by spectrophotometry

- Quantification by fluorimetry

- Quantification by the trichromatic method

- Correction for pheophytin

- Calculation of chlorophyll concentration

- Interpretation of results and applications

- Ensuring the precision and accuracy of results

- Sources of error and ways to minimize them

- Validation and quality control procedures

In-person or in-company modality

Hours: 4h

Theoretical and practical class

Online Modality

Hours: 4h

Theoretical class

Exclusive live classes​​

Target audience: Students, researchers, professionals seeking to improve and update themselves, laboratories that want to implement new analyzes in their scope, laboratories that seek accreditation with INMETRO or that wish to train their employees.

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